Journal: Cellular and Molecular Bioengineering

Article Title: Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation

doi: 10.1007/s12195-019-00574-3

Figure Lengend Snippet: HGF induces ambiguous arterio-venous marker expression. (a) HUVECs were treated with either HGF or VEGF (arterial gene inducer, control). RNA was isolated at different time points post treatment. HGF induced expression of arterial and venous genes Hey1, Coup-tfII, EphrinB2, EphB4, and Dll4 while VEGF induced expression of arterial genes Hey1, EphrinB2, and Dll4 only (mean ± SEM; n = 7; one-way ANOVA, Dunnett’s test, *p ≤ 0.05 vs. 0 h). (b) To confirm co-expression on the single-cell level, HUVECs were stimulated with HGF or VEGF for 12 h, fixed, and stained with both anti-Ephrin-B2 and anti-Eph-B4 antibodies. Flow cytometry analysis showed that HGF increased the co-expression of Eph-B4 and Ephrin-B2 in the same endothelial cell compared to VEGF, and untreated conditions (mean ± SEM; n = 3; one-way ANOVA, Bonferroni correction, p ≤ 0.01 of HGF vs. VEGF or untreated control).

Article Snippet: HUVECs treated with 100 ng/ml of VEGF (293-VE, R&D Systems) or HGF (294-HGN, R&D Systems) for 12 h were washed with PBS and detached with TrypLE (GIBCO, Thermo Fisher Scientific).

Techniques: Marker, Expressing, Control, Isolation, Staining, Flow Cytometry

Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: Effects of HGF regulated by autophagy on antifibrotic function of fPMSCs. The conditioned medium from sh-NC and sh-ATG5 infection of fPMSCs were infected with sh-NC and sh-ATG5 lentivirus and pretreated with 20 ng/mL TGF-β1 in order to prepare conditioned medium, then which was conducted to culture MRC-5 cells stimulated with 2.5 ng/mL TGF-β1 for 24 h. 500 ng/mL recombinant human HGF (rhHGF) and 1 μg/mL HGF neutralization antibody (naHGF) was added into conditioned medium respectively to interrupt the function of HGF. Protein levels of Collagen I, α-SMA and CTGF were detected by western blotting. Densitometric analyses of Collagen I, α-SMA and CTGF. Representative data from three independent experiments are shown. * P < 0.05.

Article Snippet: A coculture system was treated with 500 ng/mL recombinant human HGF (Proteintech, China) and 1 μg/mL HGF neutralization antibody (R&D Systems, USA) to verify the antifibrotic function of HGF derived from fPMSCs on MRC-5 cells.

Techniques: Infection, Recombinant, Neutralization, Western Blot